Bacterial biofilm reduction by 275 and 455 nm light pulses emitted from light emitting diodes

Eradication of biofilms from the food contact surfaces is a challenging task, owing to their increased resistance sanitizers and regular cleaning practices. The treatment with light pulses emitted emitting diode (LED) an emerging surface decontamination technology, that can produce antibiofilm effect by photodynamic inactivation. objective this study was understand efficacy 275 (Ultraviolet-C [UV-C]) 455 nm (Blue) LEDs against single dual-species Salmonella Typhimurium ATCC13311 Aeromonas australiensis 03-09 on stainless steel (SS) coupons formed at different time. biofilm formation S. improved when grown A. in culture. Both showed significant activity biofilms. For instance, LED SS 1.8 J/cm2 dose each surface, produced reductions 4.24 3.9 log (CFU/cm2) (cell attachment) Typhimurium, 4.45 4.99 australiensis. However, susceptibility toward treatments influenced presence biofilm. confocal laser scanning microscopy images revealed cell membrane damage pulses. Overall, several factors like temperature increase, strains used, dose, time, incubation period inactivation coupons. This provides insight into bacterial grade surfaces.